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Fotoiniciovaná degradace vybraných léčiv (dexametazonu, prednisolonu, fluoxetinu) a testování toxicity léčiv a produktů jejich fotodegradace na vodní organismy (\kur{Daphnia magna})
DOKOUPILOVÁ, Eliška
This diploma thesis investigates kinetics of photoinitiated degradation of selected drugs (dexamethasone, prednisolone, fluoxetine) under conditions relevant to surface waters and provides results of toxicity tests of original compounds and their photodegradation products mixtures for a representant of aquatic organisms, cladoceran Daphnia magna. The theoretical part describes the basic characteristics, mechanism of action and therapeutic usage of selected drugs. Toxicological studies related to adverse effects of these substances on aquatic organisms are also briefly outlined. The experimental part presents first the results of photochemical degradation kinetics of the studied compounds and second the findings of chronic toxicity tests of the selected drugs and their photoproducts mixtures on Daphnia magna, namely on the number of juveniles, on the number of clutches and on the body size.
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Embryotoxicity testing of psychopharmacs using the CHEST method
Pavlovič, Ondřej ; Peterka, Miroslav (advisor) ; Maňáková, Eva (referee)
Psychotropic drugs are commonly used group of pharmaceuticals, their main effect is to alter psychic condition, including mental diseases treatment. Symptoms of mental illnesses are more and more common, theref orenumber of patients diagnosed with mental illnes, and thus using psychotropics, is growing stronger. But using psychotropics during gestation is not without risks for mother and embryo itself. However, thanks to the absence of controlled human studies, the knowledge of emrbyotoxic effects of pschotropics is limited to casuistics, reported side effects and animal experimental studies. Many of those studies suggests emrbyotoxic potential of psychotropic drugs, on the other hand, others claim their safety. The goal of this thesis is to test at least some of them, using CHEST method, that allows us to observe direct effect of unmetabolized substance on chick embryo. In this thesis we tested selected psychotropics, very common antidepressant fluoxetine (prozac) and antipsychotic drug olanzapine, for embryotoxicity, using in ovo method CHEST with chick embryos as model organism. By bypassing the maternal organism and his metabolism, this method allows to observe direct effect of unmetabolized substance on chick embryo. Results revealed embryotoxic effect of fluoxetin in dosage 10-2 and 10-3 on 3rd and...
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Embryotoxicity testing of psychopharmacs using the CHEST method
Pavlovič, Ondřej ; Peterka, Miroslav (advisor) ; Maňáková, Eva (referee)
Psychotropic drugs are commonly used group of pharmaceuticals, their main effect is to alter psychic condition, including mental diseases treatment. Symptoms of mental illnesses are more and more common, theref orenumber of patients diagnosed with mental illnes, and thus using psychotropics, is growing stronger. But using psychotropics during gestation is not without risks for mother and embryo itself. However, thanks to the absence of controlled human studies, the knowledge of emrbyotoxic effects of pschotropics is limited to casuistics, reported side effects and animal experimental studies. Many of those studies suggests emrbyotoxic potential of psychotropic drugs, on the other hand, others claim their safety. The goal of this thesis is to test at least some of them, using CHEST method, that allows us to observe direct effect of unmetabolized substance on chick embryo. In this thesis we tested selected psychotropics, very common antidepressant fluoxetine (prozac) and antipsychotic drug olanzapine, for embryotoxicity, using in ovo method CHEST with chick embryos as model organism. By bypassing the maternal organism and his metabolism, this method allows to observe direct effect of unmetabolized substance on chick embryo. Results revealed embryotoxic effect of fluoxetin in dosage 10-2 and 10-3 on 3rd and...
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